BIORISM HYGIENICS TEXTILE
SCIENTIFIC EVIDENCE

Background

Biorism™ Hygienics Textile is a cutting-edge fabric protection system that integrates proprietary nanotechnology to deliver long-lasting antimicrobial performance. Developed in Europe after years of research, it is designed to protect textiles from a broad spectrum of pathogens, including bacteria, viruses, fungi, spores, and mildew. The patented nano-carrier technology binds active antimicrobial agents securely to textile fibers, providing Hygienics coverage that remains effective for days even after multiple washes or extended use.Its versatility allows application to both porous and non-porous materials, making it ideal for apparel, linens, uniforms, medical textiles, and hygienesensitive fabrics. With an eco-sensitive and biocompatible formulation, it is safe for adults, children, and pets, offering protection lasting 48 hours up to 7 days depending on use conditions.

What’s inside Biorism Hygienics Textile?

At the heart of Biorism™ Hygienics Textile is a patented nanotechnology platform developed under strict EU standards. This system uses ultra-fine nano-carrier particles engineered to firmly bond with textile fibers, creating a durable antimicrobial shield. These carriers ensure controlled, sustained release of active agents for continuous protection while preserving the softness, breathability, and comfort of the fabric. The antimicrobial formula incorporates a synergistic blend of ethanol, hydrogen peroxide, zinc chloride, and zinc nitrate. Together, these agents deactivate a wide range of harmful microorganisms upon contact, including bacteria, viruses, and fungi, while helping to prevent odors and microbial growth. Proven through laboratory testing, Sil2U® technology provides up to 99.99% pathogen reduction and maintains performance across multiple wash cycles. Its combination of advanced nanotechnology, powerful actives, and long-lasting efficacy makes Biorism™ Hygienics Textile a high-value solution for cleaner, safer, fresher fabrics in everyday and professional applications.

Scientific Evidence

To ensure the quality and safety of Biorism™ Hygienics Textile, scientific tests were conducted with Universiti Teknologi MARA (UiTM), and Success Gadget Portugal.

1. Antibacterial Test: This test was conducted to assess the ability of the treated fabric to inhibit or eliminate bacterial growth, thereby verifying its antimicrobial efficacy during intended use.

2. Dust Mite Test: This test evaluates the ability of treated textiles to inhibit the growth and establishment of common house dust mites.

3. Sporicidal Activity: This test was assessed to measure its ability to kill spores. The product was mixed with a spore solution, then surviving spores were counted to calculate its effectiveness.

4. Dermatological Test: The test material was applied repeatedly using skin patches, and reactions were monitored to confirm it is safe and non-irritating.

5. Field Emission Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy (FESEM-EDX): This test was carried out to observe the surface structure of the fabric. It also identified the elements present in the coating, such as nano-silica that carry the key ingredients.

6. Fourier Transform Infrared Spectroscopy (FTIR): FTIR identifies and verifies the chemical bonds and functional groups present in the fabric. This ensures that the intended active compounds are successfully integrated into the textile.

7. X-Ray Diffraction Spectroscopy (XRD): XRD determines the crystalline structure and phase composition of the nano-silica and other active components in the coating. It helps verify the stability and uniform distribution of the functional materials within the textile.

1. Antibacterial (Uni Minho)

The antibacterial activity of textile products was evaluated according to the international standard EN ISO 20743:2013. The test aimed to determine the antibacterial performance of textile samples treated with the product against Staphylococcus aureus (ATCC 6538) and Klebsiella pneumoniae (ATCC 11296) under controlled laboratory conditions for 24 hours.

All samples treated with Biorism Hygienics Textile at different times of application show strong antibacterial activity, based on EN ISO 20743:2021, during 24 hours of contact, for all bacteria tested.

2. Antibacterial

To further assess the antimicrobial efficacy of the treated fabric after 40 wash cycles and 30 days of use, its antibacterial activity was tested following the JIS L 1902:2008 standard. This method provides a quantitative assessment of antibacterial activity, distinguishing between bacteriostatic (growth inhibition) and bactericidal (killing) effects. Treated and untreated fabric swatches are inoculated with test organisms, incubated, and then bacteria are eluted into a neutralizing solution. The bacterial count is determined, and the percentage reduction relative to the control indicates the antibacterial performance of the fabric.

Staphylococcus aureus (ATCC 12228)

Escherichia coli (CECT 434)

Candida albicans (SC 5314)

Conclusion

The treated textile demonstrated excellent antibacterial performance, maintaining microbial reduction even after extended washing and storage, indicating durable and long-lasting antimicrobial efficacy.

3. Dust Mite Test

The AATCC 194 method was used to assess the ability of Biorism™ Hygienics Textile to prevent dust mite infestation. Treated fabric was tested against common dust mite species (Dermatophagoides farinae and Dermatophagoides pteronyssinus) under longterm conditions, with results compared to untreated control samples.

Conclusion

No dust mite colonies were established on treated samples under both unwashed and long-term aged conditions, including after multiple wash cycles, indicating 100% reduction in mite count. This performance suggests the treatment provides durable protection over extended periods and usage conditions, making it highly effective for applications such as mattress and pillow encasings to benefit individuals with asthma or dust mite allergies.

4. Sporicidal Activity

The sporicidal activity was evaluated using the EN 13704:2018 quantitative suspension test, designed for chemical disinfectants in food, industrial, domestic, and institutional areas (Phase 2, Step 1). In this method, a sample of the product is mixed with a suspension of bacterial spores in the presence of an interfering substance and maintained for a specified contact time and temperature. At the end of the contact period, the mixture is neutralized using a validated method. Surviving spores are counted to determine the percentage reduction, confirming the fabric’s effectiveness against spore-forming microorganisms.

Result

For the microorganism Bacillus subtilis, the product achieved a reduction greater than 10³ at a concentration of 8% under the conditions specified in EN 13704:2018, demonstrating effective sporicidal activity.

Conclusion

The product exhibited strong sporicidal activity against Bacillus subtilis, achieving a reduction greater than 10³ at an 8% concentration. These findings, confirm its effectiveness in inactivating spore-forming microorganisms under the tested conditions.

5. Dermatological Tests

This study aimed to assess the irritation and sensitisation potential of the test article on healthy volunteers using repeated cutaneous patch applications, following the modified Draize method of Jordan and King (1977). The evaluation supports product claims such as “Dermatologically Tested,” “Clinically Tested,” “Kind to Skin,” and “Safe to Skin.”

The study was conducted as a single-blind trial in one centre with 108 volunteers who met inclusion and exclusion criteria and provided informed consent. A Scanpor® tape with an attached Finn chamber was used to apply approximately 0.02 g or 0.02 mL of the test article onto small filter papers for skin contact evaluation. Crystal violet markers ensured accurate patch placement, and participants were instructed to keep patches dry and avoid disturbing the dye marks.

Conclusion

Under the conditions of this study, the test article was well-tolerated and showed no signs of irritation or sensitisation. These findings confirm that the product is safe for use and substantiate claims such as “Dermatologically Tested,” “Clinically Tested,” “Kind to Skin,” and “Safe to Skin.”

6. FESEM Analysis

Untreated fabric
The untreated fabric showed an irregular weave with many detached fibres and a smooth surface, indicating no coating or reinforcement, making it more vulnerable to mechanical or microbial attack.

Figure 1: Untreated fabric for the magnification of a) 250× b) 4000× and c) 8000×

Treated fabric with Biorism Hygienics Textile

Fabric treated with Biorism Hygienics Textile had a patchy, uneven surface with some fibre pull-outs, but the coating adhered well in several areas and reduced gaps between fibres. The increased roughness and silica particles likely help prevent bacterial attachment, providing effective antibacterial protection despite the non-uniform coating.

Figure 2: Treated fabric with Biorism Hygienics Textile with the magnification of a)

250× b) 4000× and c) 8000×

Element Analysis (EDX)

Table 1: Elemental analysis of untreated and treated fabric with Hygienics Textile.

Carbon – Remained similar to untreated fabric, reflecting the base organic composition of Tencel fibres.

Oxygen – Slightly decreased, consistent with partial coverage of the surface by silica.

Sodium – Detected at 1.50% in treated fabric, likely from processing or treatment agents.

Silicon – Increased from 0.23% in untreated fabric to 0.64% in treated fabric, confirming successful deposition of silica on the fibre surface.

Conclusion

The untreated fabric exhibited an irregular weave, visible fibre detachments, and a smooth, unprotected surface, indicating low resistance to mechanical or microbial damage. In contrast, the treated fabric showed a patchy but well-adhered silica coating in several areas, reducing fibre gaps and increasing surface roughness features known to inhibit bacterial attachment. EDX analysis confirmed the presence of silicon on the fabric surface, indicating successful silica deposition and surface functionalisation. Despite some coating non-uniformity and minor particle aggregation, the treatment provided enhanced antibacterial potential as proven above and improved protective properties compared to the untreated fabric.

7. X-Ray Diffraction Spectroscopy (XRD)

Figure 1: XRD diffractogram of a) untreated fabric and b) treated fabric with Biorism Hygienics Textile

Untreated Fabric

1. Displayed characteristic crystalline cellulose peaks at 12.3°, 20.5°, and 21.7°.

2. No significant additional phases were detected, confirming its natural cellulose structure.

Treated Fabric with Biorism Hygienics Textile

1. Retained the same cellulose crystalline peaks as the untreated sample, confirming preservation of the base fabric structure.

2. Slight reduction in peak intensity and minor peak convolution observed, likely due to silica nanoparticle deposition.

3. Absence of sharp quartz peaks indicated the silica was amorphous and nano-sized, beneficial for functional performance.


Conclusion

The XRD analysis confirms that treatment with Biorism Hygienics successfully deposits amorphous silica nanoparticles onto the fabric without altering its fundamental cellulose structure. The amorphous nature of silica contributes to improved surface functionality, such as enhanced antibacterial resistance and durability, while maintaining the flexibility and integrity of the original fabric.

8. Fourier Transform Infrared Spectroscopy (FTIR)

Table 1: Summary of FTIR spectra for untreated fabric and treated fabric with Biorism Hygienics Textile.

Figure 1: FTIR spectra of untreated fabric and treated fabric with Biorism Hygienics Textile. 

The FTIR spectra confirmed the characteristic cellulose peaks in untreated fabric, including O–H stretching, C–H stretching, O–H bending, CH₂ vibrations, and C–O–C/C=O stretching, consistent with its natural lyocell composition. Upon treatment with Biorism™ 360, notable spectral changes were observed. The reduction in peak intensity at ~2900, ~1640, and ~1425 cm⁻¹ indicates the effect of surface coating on cellulose functional groups. A decrease in intensity within the ~1160–1030 cm⁻¹ range suggests overlap with Si–O–C absorption. Most importantly, the appearance of a new band at ~800–780 cm⁻¹ corresponds to Si–O–Si stretching, confirming the successful deposition of silica nanoparticles on the fabric surface.


Conclusion

FTIR analysis validates that the fabric retains its cellulose structure after treatment while incorporating silica nanoparticles. The presence of Si–O–Si peaks confirm effective surface functionalisation, demonstrating that Biorism™ Hygienics Textile treatment enhances the fabric with additional functional properties without compromising its cellulose integrity.

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